Cart 2

P5CS antibody

Cat.#: 113555

Size:

Special Price 239.0 USD

Availability: In Stock
- +

Add to cart to get an online quotation

Product Information

  • Product Name
    P5CS antibody
  • Documents
  • Description
    P5CS Rabbit Polyclonal antibody. Positive WB detected in MCF7 cells, HeLa cells, HepG2 cells, mouse brain tissue. Positive IP detected in mouse brain tissue. Observed molecular weight by Western-blot: 87 kDa
  • Tested applications
    ELISA, WB, IP
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    ALDH18A1 antibody; GPR antibody; GSAS antibody; P5CS antibody; PYCS antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of P5CS recombinant protein (Accession Number: NM_002860). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:200-1:2000

  • Validations

    MCF7 cells were subjected to SDS PAGE followed by western blot with Catalog No:113555(ALDH18A1 antibody) at dilution of 1:500

    MCF7 cells were subjected to SDS PAGE followed by western blot with Catalog No:113555(ALDH18A1 antibody) at dilution of 1:500

    IP Result of anti-ALDH18A1 (IP:Catalog No:113555, 3ug; Detection:Catalog No:113555 1:500) with mouse brain tissue lysate 5000ug.

    IP Result of anti-ALDH18A1 (IP:Catalog No:113555, 3ug; Detection:Catalog No:113555 1:500) with mouse brain tissue lysate 5000ug.

  • Background
    Delta 1-pyrroline-5-carboxylate synthetase (P5CS), also named as GSAS, P5CS, PYCS, catalyzes the ATP and the NAD(P)H-dependent conversion of L-glutamate to glutamic gamma-semialdehyde (GSA) which is the metabolic precursor for proline biosynthesis(PMID:8761662). This protein includes 2 domains: glutamate 5-kinase and gamma-glutamyl phosphate reductase. In the N-terminal section, it belongs to the glutamate 5-kinase family and in the C-terminal section, it belongs to the gamma-glutamyl phosphate reductase family. Defects in ALDH18A1 are the cause of cutis laxa, autosomal recessive, type 3A (ARCL3A)(PMID:18478038). It has 2 isoforms produced by alternative splicing.
  • References
    • Liu W, Hancock CN, Fischer JW, Harman M, Phang JM. Proline biosynthesis augments tumor cell growth and aerobic glycolysis: involvement of pyridine nucleotides. Scientific reports. 5:17206. 2015.
    • Liu W, Le A, Hancock C. Reprogramming of proline and glutamine metabolism contributes to the proliferative and metabolic responses regulated by oncogenic transcription factor c-MYC. Proceedings of the National Academy of Sciences of the United States of America. 109(23):8983-8. 2012.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"