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Product Name
p35 antibody
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Description
p35 Rabbit Polyclonal antibody. Positive IP detected in mouse brain tissue. Positive WB detected in mouse brain tissue, rat brain tissue. Observed molecular weight by Western-blot: 35 kDa
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Tested applications
ELISA, WB, IP
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Species reactivity
Human, Mouse, Rat; other species not tested.
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Alternative names
CDK5 activator 1 antibody; CDK5P35 antibody; CDK5R antibody; CDK5R1 antibody; NCK5A antibody; p23 antibody; p25 antibody; p35 antibody; p35nck5a antibody; TPKII regulatory subunit antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of p35 recombinant protein (Accession Number: XM_011525438). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:113548(CDK5R1 antibody) at dilution of 1:600
IP Result of anti-CDK5R1 (IP:Catalog No:113548, 4ug; Detection:Catalog No:113548 1:500) with mouse brain tissue lysate 3440ug.
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Background
Cyclin dependent kinase-5 (Cdk5), is an atypical but essential member of the Cdk family of proline-directed serine/threonine kinases. CDK5R1 encodes p35, a specific activator of the serine/threonine kinase CDK5, which plays a central role in neuronal migration and differentiation during central nervous system development. The p35 form of this protein is proteolytically cleaved by calpain, generating a p25 form.
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