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P27; KIP1 antibody

Cat.#: 113541

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Product Information

  • Product Name
    P27; KIP1 antibody
  • Documents
  • Description
    P27; KIP1 Rabbit Polyclonal antibody. Positive WB detected in NIH/3T3 cells, HepG2 cells. Positive IP detected in NIH/3T3 cells. Observed molecular weight by Western-blot: 27 kDa
  • Tested applications
    ELISA, WB, IP
  • Species reactivity
    Human, Mouse; other species not tested.
  • Alternative names
    CDKN1B antibody; CDKN4 antibody; KIP1 antibody; MEN1B antibody; MEN4 antibody; P27 antibody; P27; KIP1 antibody; P27KIP1 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of P27; KIP1 recombinant protein (Accession Number: NM_004064). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IP: 1:200-1:1000

  • Validations

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with Catalog No:113541(P27; KIP1 Antibody) at dilution of 1:600

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with Catalog No:113541(P27; KIP1 Antibody) at dilution of 1:600

    IP Result of anti-P27; KIP1 (IP:Catalog No:113541, 4ug; Detection:Catalog No:113541 1:300) with NIH/3T3 cells lysate 1600ug.

    IP Result of anti-P27; KIP1 (IP:Catalog No:113541, 4ug; Detection:Catalog No:113541 1:300) with NIH/3T3 cells lysate 1600ug.

  • Background
    CDKN1B, also named as P27 or KIP1, is a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. P27 binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controlling cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Downregulation of P27 has been implicated in the progression of several malignancies, including lung cancer, hepatocellular carcinoma, salivary cancer, oral squamous cell carcinomas, and gastric cancer.
  • References
    • Xu D, Wang J, Zhou Z, He Z, Zhao Q. Cannabinoid WIN55, 212-2 induces cell cycle arrest and inhibits the proliferation and migration of human BEL7402 hepatocellular carcinoma cells. Molecular medicine reports. 12(6):7963-70. 2015.
    • Yin J, Sheng B, Han B. The AhR is involved in the regulation of LoVo cell proliferation through cell cycle-associated proteins. Cell biology international. 2016.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"