Product Name

P21;CDKN1A antibody

Documents

Description

P21;CDKN1A Rabbit Polyclonal antibody. Positive FC detected in HeLa cells. Positive IHC detected in human kidney tissue, human breast cancer tissue, rat brain tissue. Positive IF detected in myotome of a somite at E11.5 tissue, MCF-7 cells. Positive IP detected in MCF-7 cells. Positive WB detected in MCF-7 cells, HEK-293 cells, HeLa cells, HUVEC cells. Observed molecular weight by Western-blot: 21 kDa

Tested applications

ELISA, IF, WB, IHC, FC, IP

Species reactivity

Human,Mouse,Rat; other species not tested.

Alternative names

CAP20 antibody; CDK interacting protein 1 antibody; CDKN1 antibody; CDKN1A antibody; CIP1 antibody; MDA 6 antibody; MDA6 antibody; P21 antibody; p21CIP1 antibody; PIC1 antibody; SDI1 antibody; WAF1 antibody

Isotype

Rabbit IgG

Preparation

This antibody was obtained by immunization of P21;CDKN1A recombinant protein (Accession Number: NM_001220777). Purification method: Antigen affinity purified.

Clonality

Polyclonal

Formulation

PBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Storage instructions

Store at -20℃. DO NOT ALIQUOT

Applications

Recommended Dilution:

WB: 1:500-1:5000

IP: 1:200-1:1000

IHC: 1:20-1:200

IF: 1:20-1:200

Validations

MCF-7 cells were subjected to SDS PAGE followed by western blot with Catalog No:113540(P21;CDKN1A Antibody) at dilution of 1:1000

Immunofluorescent analysis of (10% Formaldehyde) fixed MCF-7 cells using Catalog No:113540(P21;CDKN1A Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

IF result of anti-p21 (CDKN1A, Catalog No:113540) in the myotome of a somite at E11.5 by Dr. Zalc A. and Dr. Relaix F. (Red = p21; Green=Pax3 from a GFP reporter; Bleu=DAPI)

Immunohistochemical of paraffin-embedded human kidney using Catalog No:113540(P21 antibody) at dilution of 1:50 (under 10x lens)

IP Result of anti-P21;CDKN1A (IP:Catalog No:113540, 4ug; Detection:Catalog No:113540 1:300) with MCF-7 cells lysate 3200ug.

Immunohistochemical of paraffin-embedded human kidney using Catalog No:113540(P21 antibody) at dilution of 1:50 (under 40x lens)

1X10^6 HeLa cells were stained with 0.2ug P21;CDKN1A antibody (Catalog No:113540, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

Background

CDKN1A (p21, CIP1, WAF1) is a cyclin-dependent kinase inhibitor. CDKN1A binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at the G1 phase. The expression of CDKN1A is induced by wild-type but not mutant p53 protein, through which CDKN1A mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. CDKN1A can interact with proliferating cell nuclear antigen (PCNA), and plays a regulatory role in S phase DNA replication and DNA damage repair. CDKN1A was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Two alternatively spliced variants, which encode an identical protein, have been reported.

References

Deng S, Tang S, Dai C. P21(Waf1/Cip1) plays a critical role in furazolidone-induced apoptosis in HepG2 cells through influencing the caspase-3 activation and ROS generation. Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association. 88:1-12. 2016.
Chu K, Gao G, Yang X. miR-512-5p induces apoptosis and inhibits glycolysis by targeting p21 in non-small cell lung cancer cells. International journal of oncology. 48(2):577-86. 2016.
Fan Z, Cui H, Xu X. MiR-125a suppresses tumor growth, invasion and metastasis in cervical cancer by targeting STAT3. Oncotarget. 6(28):25266-80. 2015.
Zhang J, Sun Q, Zhang Z, Ge S, Han ZG, Chen WT. Loss of microRNA-143/145 disturbs cellular growth and apoptosis of human epithelial cancers by impairing the MDM2-p53 feedback loop. Oncogene. 32(1):61-9. 2013.
Yao Y, Li L, Zhang H. Enhanced therapeutic efficacy of vitamin K2 by silencing BCL-2 expression in SMMC-7721 hepatocellular carcinoma cells. Oncology letters. 4(1):163-167. 2012.
Cun B, Song X, Jia R. Cell growth inhibition in HPV 18 positive uveal melanoma cells by E6/E7 siRNA. Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine. 34(3):1801-6. 2013.
Xu X, Hueckstaedt LK, Ren J. Deficiency of insulin-like growth factor 1 attenuates aging-induced changes in hepatic function: role of autophagy. Journal of hepatology. 59(2):308-17. 2013.
Wu H, Xiao Y, Zhang S. The Ets transcription factor GABP is a component of the hippo pathway essential for growth and antioxidant defense. Cell reports. 3(5):1663-77. 2013.

P21;CDKN1A antibody

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