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Product Name
p130Cas antibody
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Description
p130Cas Rabbit Polyclonal antibody. Positive IP detected in RAW 264.7 cells. Positive WB detected in A431 cells, COLO 320 cells. Positive IF detected in Hela cells. Positive IHC detected in human colon cancer tissue. Observed molecular weight by Western-blot: 116 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
BCAR1 antibody; CAS antibody; CAS1 antibody; CASS1 antibody; CRK assOCiated substrate antibody; CRKAS antibody; p130Cas antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of p130Cas recombinant protein (Accession Number: NM_014567). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
A431 cells were subjected to SDS PAGE followed by western blot with Catalog No:113534(BCAR1 antibody) at dilution of 1:800
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:113534(BCAR1 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:113534(BCAR1 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of Hela cells, using BCAR1 antibody Catalog No:113534 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-BCAR1 (IP:Catalog No:113534, 4ug; Detection:Catalog No:113534 1:600) with RAW 264.7 cells lysate 4000ug.
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Background
BCAR1 represents breast cancer anti-estrogen resistance 1
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"