P110 peptide

P110 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

H-YGRKKRRQRRRGGDLLPRGS-NH2

H-Tyr-Gly-Arg-Lys-Lys-Arg-Arg-Gln-Arg-Arg-Arg-Gly-Gly-Asp-Leu-Leu-Pro-Arg-Gly-Ser-NH2

20

95.66%

C₁₀₀H₁₇₉N₄₅O₂₅

2411.77

Synthetic

Powder

P110 inhibits Drp1 enzyme activity and blocks the Drp1/Fis1 (Dynamin related protein 1/fission 1) interaction in vitro and in cultured neurons, but has no effect on the interaction between Drp1 and other mitochondrial adaptors. In Parkinson's disease models P110 is neuroprotective by inhibiting mitochondrial fragmentation and reactive oxygen species (ROS) production and subsequently improving mitochondrial membrane potential and mitochondrial integrity. P110 increases neuronal cell viability by reducing apoptosis and autophagic cell death, and reduces neurite loss of primary dopaminergic neurons.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual:Handling and Storage of Synthetic Peptides

• Qi et al (2013) A novel Drp1 inhibitor diminishes aberrant mitochondrial fission and neurotoxicity. J.Cell Sci. 126 789 PMID: 23239023
• Joshi et al (2018) Drp1/Fis1 interaction mediates mitochondrial dysfunction, bioenergetic failure and cognitive decline in Alzheimer's disease. Oncotarget 9 6128 PMID: 29464060
• Iqbal et al (2018) A synthetic peptide rescues rat cortical neurons from anesthetic-induced cell death, perturbation of growth and synaptic assembly. Sci Rep 11 4567 PMID: 33633281

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.