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Product Name
OSCP antibody
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Description
OSCP Rabbit Polyclonal antibody. Positive FC detected in HepG2 cells. Positive IF detected in HepG2 cells. Positive IHC detected in human heart tissue, human colon cancer tissue, human testis tissue. Positive WB detected in HEK-293 cells, human kidney tissue, human placenta tissue, mouse colon tissue, mouse heart tissue, mouse liver tissue, mouse placenta tissue. Observed molecular weight by Western-blot: 23-25kd
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Tested applications
ELISA, WB, IHC, IF, FC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ATP5O antibody; ATPO antibody; OSCP antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of OSCP recombinant protein (Accession Number: NM_001697). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:113435(ATP5O antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human heart using Catalog No:113435(ATP5O antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human heart using Catalog No:113435(ATP5O antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using ATP5O antibody Catalog No:113435 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
1X10^6 HepG2 cells were stained with 0.2ug ATP5O antibody (Catalog No:113435, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
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Background
ATP5O(ATP synthase subunit O, mitochondrial) is also named as ATPO, OSCP and belongs to the ATPase delta chain family. The gene encodes the oligomycin sensitivity-conferring protein (OSCP) of ATP synthase. The predicted polypeptide is 213 amino acids long with more than 80% identity to the bovine and mouse homologs and it is expressed at highest levels in muscle and heart by the northern blot(PMID:7490082). Many studies have indicated that mitochondrial dysfunction is central to the development of most age-related human diseases including neurodegenerative diseases, cancer, and type 2 diabetes.
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