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OS9 antibody

Cat.#: 113427

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Product Information

  • Product Name
    OS9 antibody
  • Documents
  • Description
    OS9 Rabbit Polyclonal antibody. Positive IHC detected in human lung cancer. Positive WB detected in HEK-293 cells, HeLa cells. Observed molecular weight by Western-blot: 73kd
  • Tested applications
    ELISA, WB, IHC
  • Species reactivity
    human; other species not tested.
  • Alternative names
    OS9 antibody; Amplified in osteosarcoma 9 antibody; OS 9 antibody; Protein OS 9 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of OS9 recombinant protein (Accession Number: NM_001017956). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:1000

    IHC: 1:20-1:200

  • Validations

    HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:113427(OS9 antibody) at dilution of 1:300

    HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:113427(OS9 antibody) at dilution of 1:300

    Immunohistochemical of paraffin-embedded human lung cancer using Catalog No:113427(OS9 antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemical of paraffin-embedded human lung cancer using Catalog No:113427(OS9 antibody) at dilution of 1:50 (under 10x lens)

  • Background
    OS-9 is a ubiquitously expressed ensoplasmic reticulum (ER)-associated protein originally identified as being amplified in certain osteosarcomas. It functions in ER quality control and ER-associated degradation (ERAD). There are three isoforms of this protein: OS-9.1, OS-9.2 and OS-9.3. The longest isoform, OS-9.1, contains 667 aa, OS-9.2 lacks aa 535-589, whereas OS-9.3 lacks aa 456-470 and 535-589. OS-9.1 and OS-9.2 are N-glycosylated, ubiquitously expressed in human tissues, and amplified in tumors. Isoform 2 is the major isoform detected in all cell types examined. This antibody can recognize all three isoforms of OS-9.
  • References
    • Christianson JC, Shaler TA, Tyler RE, Kopito RR. OS-9 and GRP94 deliver mutant alpha1-antitrypsin to the Hrd1-SEL1L ubiquitin ligase complex for ERAD. Nature cell biology. 10(3):272-82. 2008.
    • Wang Y, Fu X, Gaiser S. OS-9 regulates the transit and polyubiquitination of TRPV4 in the endoplasmic reticulum. The Journal of biological chemistry. 282(50):36561-70. 2007.
    • Fujimori T, Kamiya Y, Nagata K, Kato K, Hosokawa N. Endoplasmic reticulum lectin XTP3-B inhibits endoplasmic reticulum-associated degradation of a misfolded α1-antitrypsin variant. The FEBS journal. 280(6):1563-75. 2013.
    • Iida Y, Fujimori T, Okawa K, Nagata K, Wada I, Hosokawa N. SEL1L protein critically determines the stability of the HRD1-SEL1L endoplasmic reticulum-associated degradation (ERAD) complex to optimize the degradation kinetics of ERAD substrates. The Journal of biological chemistry. 286(19):16929-39. 2011.
    • Hosokawa N, Kamiya Y, Kamiya D, Kato K, Nagata K. Human OS-9, a lectin required for glycoprotein endoplasmic reticulum-associated degradation, recognizes mannose-trimmed N-glycans. The Journal of biological chemistry. 284(25):17061-8. 2009.
    • Hosokawa N, Wada I, Nagasawa K, Moriyama T, Okawa K, Nagata K. Human XTP3-B forms an endoplasmic reticulum quality control scaffold with the HRD1-SEL1L ubiquitin ligase complex and BiP. The Journal of biological chemistry. 283(30):20914-24. 2008.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"