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Product Name
ODF2 antibody
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Description
ODF2 Rabbit Polyclonal antibody. Positive IHC detected in human breast cancer tissue. Positive IF detected in HeLa cells. Positive IP detected in mouse testis tissue. Positive WB detected in mouse testis tissue, mouse lung tissue. Observed molecular weight by Western-blot: 67-100 kDa
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Tested applications
ELISA, WB, IHC, IP, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Cenexin antibody; FLJ44866 antibody; ODF2 antibody; ODF2/1 antibody; ODF2/2 antibody; ODF84 antibody; Outer dense fiber protein 2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ODF2 recombinant protein (Accession Number: BC010629). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
mouse testis tissue were subjected to SDS PAGE followed by western blot with Catalog No:113325(ODF2 antibody) at dilution of 1:500
IP Result of anti-ODF2 (IP:Catalog No:113325, 5ug; Detection:Catalog No:113325 1:500) with mouse testis tissue lysate 8000ug.
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:113325(ODF2 antibody) at dilution of 1:50 (under 10x lens)
Immunofluorescent analysis of HeLa cells using Catalog No:113325(ODF2 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
ODF2 (outer dense fiber 2) is the major cenexin protein of the cytoskeleton of the sperm tail. In somatic cells, it is a component of the centrosome in which it is located in the appendages of the mother centriole. The gene for the cenexin protein, which was first identified as a protein marker of the mother centriole, was subsequently found to be identical to ODF2 and encodes transcripts cenexin 1 and cenexin 2. ODF2 is ubiquitously expressed in many cell types as various splicing variants. Additionally, ODF2 localizes to centrioles, basal bodies, and primary cilia, which are all structurally and functionally interconnected. ODF2 has 10 isoforms with MW 67-100 kDa.
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References
- Wu JM, Chen CT, Coumar MS. Aurora kinase inhibitors reveal mechanisms of HURP in nucleation of centrosomal and kinetochore microtubules. Proceedings of the National Academy of Sciences of the United States of America. 110(19):E1779-87. 2013.
- Chung JJ, Shim SH, Everley RA, Gygi SP, Zhuang X, Clapham DE. Structurally distinct Ca(2+) signaling domains of sperm flagella orchestrate tyrosine phosphorylation and motility. Cell. 157(4):808-22. 2014.
- Hehnly H, Doxsey S. Rab11 endosomes contribute to mitotic spindle organization and orientation. Developmental cell. 28(5):497-507. 2014.
- Kong D, Farmer V, Shukla A. Centriole maturation requires regulated Plk1 activity during two consecutive cell cycles. The Journal of cell biology. 206(7):855-65. 2014.
- Zebrowski DC, Vergarajauregui S, Wu CC. Developmental alterations in centrosome integrity contribute to the post-mitotic state of mammalian cardiomyocytes. eLife. 4:. 2015.
- Hehnly H, Canton D, Bucko P. A mitotic kinase scaffold depleted in testicular seminomas impacts spindle orientation in germ line stem cells. eLife. 4:e09384. 2015.
- Hegan PS, Ostertag E, Geurts AM, Mooseker MS. Myosin Id is required for planar cell polarity in ciliated tracheal and ependymal epithelial cells. Cytoskeleton (Hoboken, N.J.). 72(10):503-16. 2015.
- Dai J, Xu W, Zhao X. Protein profile screening: reduced expression of Sord in the mouse epididymis induced by nicotine inhibits tyrosine phosphorylation level in capacitated spermatozoa. Reproduction (Cambridge, England). 151(3):227-37. 2016.
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