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Product Name
ODC1 antibody
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Description
ODC1 Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IHC detected in human prostate hyperplasia tissue, human placenta tissue, human prostate cancer tissue. Positive WB detected in mouse thymus tissue. Observed molecular weight by Western-blot: 53 kDa
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Tested applications
ELISA, IF, IHC, WB
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ODC antibody; ODC1 antibody; Ornithine decarboxylase antibody; ornithine decarboxylase 1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ODC1 recombinant protein (Accession Number: NM_001287189). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
Immunohistochemical of paraffin-embedded human prostate hyperplasia using Catalog No:113322(ODC1 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human prostate hyperplasia using Catalog No:113322(ODC1 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using ODC1 antibody Catalog No:113322 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
mouse thymus tissue were subjected to SDS PAGE followed by western blot with Catalog No:113322(ODC1 Antibody) at dilution of 1:300
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Background
Ornithine decarboxylase (ODC) is also named as ODC1and belongs to the Orn/Lys/Arg decarboxylase class-II family. It catalyzes the conversion of ornithine to putrescine, the first step and a major site of regulation of polyamine biosynthesis. The level of ODC is known to be controlled at several sites, namely transcription, translation, and enzyme degradation. Polyamines can stimulate the degradation of ODC as a type of negative feedback control(PMID:8486633). This protein can be phosphorylated in vivo(PMID:8798774).
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References
- Zhou R, Kang X, Tang B. Ornithine is a key mediator in hyperphosphatemia-mediated human umbilical vein endothelial cell apoptosis: Insights gained from metabolomics. Life sciences. 146:73-80. 2016.
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