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Product Name
OCT1 antibody
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Description
OCT1 Rabbit Polyclonal antibody. Positive IF detected in HeLa cells. Positive IHC detected in human lymphoma tissue, human colon cancer tissue. Positive IP detected in HeLa cells. Positive WB detected in HeLa cells, HEK-293 cells, NCCIT cell. Observed molecular weight by Western-blot: 90 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human, Pig; other species not tested.
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Alternative names
NF A1 antibody; OCt 1 antibody; OCT1 antibody; OCtamer binding protein 1 antibody; OTF 1 antibody; OTF1 antibody; POU class 2 homeobox 1 antibody; POU2F1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ℃T1 recombinant protein (Accession Number: XM_047422875). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:1000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:113469(POU2F1 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human lymphoma using Catalog No:113469(POU2F1 antibody) at dilution of 1:100 (under 25x lens)
IP Result of anti-POU2F1 (IP:Catalog No:113469, 4ug; Detection:Catalog No:113469 1:300) with HeLa cells lysate 1600ug.
Immunofluorescent analysis of HeLa cells using Catalog No:113469(POU2F1 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
The POU class 2 homeobox 1 (POU2F1), also known as octamer-binding transcription factor-1 (ocT-1), is a ubiquitous nuclear protein that regulates the transcription of target genes by binding to a cis-acting octamer element(5’-ATTTGCAT-3’). It participated in the regulation of housekeeping genes, including histone H2B and snRNAs, for cell differentiation and proliferation [PMID:3462701]. In addition, it functions as a cellular stress sensor that modulates activities of tissue-specific target genes involved in immune and inflammatory responses [PMID:16322220]. It also acts as a repressor for the cytokine-inducible, NF-kB-dependent expression of E-selectin and the vascular cell adhesion molecule during vascular inflammation. Otherwise, it can interact with nuclear hormone receptors, such as the retinoid X receptor, the thyroid hormone receptor and the glucocorticoid receptor, and influence their transcription activity[PMID:17192276].
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References
- Chen K, Huang C, Yuan J, Cheng H, Zhou R. Long-term artificial selection reveals a role of TCTP in autophagy in mammalian cells. Molecular biology and evolution. 31(8):2194-211. 2014.
- Wen J, Zeng M, Shu Y. Aging increases the susceptibility of cisplatin-induced nephrotoxicity. Age (Dordrecht, Netherlands). 37(6):112. 2015.
- Zhao M, Fan J, Liu Y. Oncogenic role of the TP53-induced glycolysis and apoptosis regulator in nasopharyngeal carcinoma through NF-κB pathway modulation. International journal of oncology. 48(2):756-64. 2016.
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