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Product Name
NXN antibody
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Description
NXN Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, human heart tissue. Positive IP detected in HeLa cells. Positive IF detected in HepG2 cells. Positive IHC detected in human testis tissue. Observed molecular weight by Western-blot: 48 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
FLJ12614 antibody; NRX antibody; nucleoredoxin antibody; NXN antibody; TRG 4 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of NXN recombinant protein (Accession Number: NM_001205319). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:113454(NXN antibody) at dilution of 1:400
Immunohistochemical of paraffin-embedded human testis using Catalog No:113454(NXN antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human testis using Catalog No:113454(NXN antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using NXN antibody Catalog No:113454 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-NXN (IP:Catalog No:113454, 4ug; Detection:Catalog No:113454 1:500) with HeLa cells lysate 3200ug.
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Background
Nucleoredoxin (NRX/NXN), a relatively uncharacterized member of the TRX family protein, has recently been reported to regulate the Wnt/β-catenin pathway, which itself regulates cell fate and early development, in a redox-dependent manner(PMID:17567240). Northern blot analysis detected several nucleoredoxin splice variants in adult mouse tissues, with highest expression in testis and skin and lowest expression in liver(PMID:9119370). It has 3 isoforms produced by alternative splicing. This antibody is specific to NXN.
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References
- Kajla S, Mondol AS, Nagasawa A. A crucial role for Nox 1 in redox-dependent regulation of Wnt-β-catenin signaling. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 26(5):2049-59. 2012.
- Hamanaka RB, Glasauer A, Hoover P. Mitochondrial reactive oxygen species promote epidermal differentiation and hair follicle development. Science signaling. 6(261):ra8. 2013.
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