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NXF1 antibody

Cat.#: 113451

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Product Information

  • Product Name
    NXF1 antibody
  • Documents
  • Description
    NXF1 Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, HEK-293 cells. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 66-70 kDa
  • Tested applications
    ELISA, WB, IF
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    DKFZp667O0311 antibody; MEX67 antibody; mRNA export factor TAP antibody; nuclear RNA export factor 1 antibody; NXF1 antibody; TAP antibody; Tip assOCiated protein antibody; Tip assOCiating protein antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of NXF1 recombinant protein (Accession Number: NM_006362). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IF: 1:20-1:200

  • Validations

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:113451(NXF1 antibody) at dilution of 1:600

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:113451(NXF1 antibody) at dilution of 1:600

    Immunofluorescent analysis of HepG2 cells using Catalog No:113451(NXF1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

    Immunofluorescent analysis of HepG2 cells using Catalog No:113451(NXF1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

  • Background
    Nuclear RNA export factor 1 (NXF1, synonyms: TAP, MEX67) is one member of a family of nuclear RNA export factors. Common domain features of this family are a noncanonical RNP-type RNA-binding domain (RBD), 4 leucine-rich repeats (LRRs), a nuclear transport factor 2 (NTF2)-like domain that allows heterodimerization with NTF2-related export protein-1(NXT1), and a ubiquitin-associated domain that mediates interactions with nucleoporins. The LRRs and NTF2-like domains are required for export activity. This protein shuttles between the nucleus and the cytoplasm and binds in vivo to poly(A)+ RNA. It is the vertebrate homologue of the yeast protein Mex67p. NXF1 overcomes the mRNA export block caused by the presence of saturating amounts of CTE (constitutive transport element) RNA of type D retroviruses.
  • References
    • Kim M, Bellini M, Ceman S. Fragile X mental retardation protein FMRP binds mRNAs in the nucleus. Molecular and cellular biology. 29(1):214-28. 2009.
    • Zhang L, Das P, Schmolke M. Inhibition of pyrimidine synthesis reverses viral virulence factor-mediated block of mRNA nuclear export. The Journal of cell biology. 196(3):315-26. 2012.
    • Stubbs SH, Conrad NK. Depletion of REF/Aly alters gene expression and reduces RNA polymerase II occupancy. Nucleic acids research. 43(1):504-19. 2015.
    • Cohen HR, Panning B. XIST RNA exhibits nuclear retention and exhibits reduced association with the export factor TAP/NXF1. Chromosoma. 116(4):373-83. 2007.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"