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Product Name
NUSAP1 antibody
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Description
NUSAP1 Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IHC detected in human colon cancer tissue, human pituitary adenoma tissue, human prostate cancer tissue. Positive WB detected in rat colon tissue, HeLa cells, mouse kidney tissue, mouse thymus tissue. Positive IP detected in HeLa cells. Observed molecular weight by Western-blot: 47-52 kDa
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Tested applications
ELISA, IHC, IF, WB, IP
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Species reactivity
Human, Mouse, Rat; other species not tested.
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Alternative names
ANKT antibody; BM037 antibody; FLJ13421 antibody; LNP antibody; NuSAP antibody; NUSAP1 antibody; PRO0310 antibody; PRO0310p1 antibody; Q0310 antibody; SAPL antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of NUSAP1 recombinant protein (Accession Number: NM_018454). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:113448(NUSAP1 antibody) at dilution of 1:50 (under 10x lens)
Immunofluorescent analysis of HepG2 cells, using NUSAP1 antibody Catalog No:113448 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
rat colon tissue were subjected to SDS PAGE followed by western blot with Catalog No:113448(NUSAP1 Antibody) at dilution of 1:1000
IP Result of anti-NUSAP1 (IP:Catalog No:113448, 4ug; Detection:Catalog No:113448 1:500) with HeLa cells lysate 2000ug.
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Background
NUSAP1, also named as ANKT, belongs to the NUSAP family. It is microtubule-associated protein with the capacity to bundle and stabilize microtubules. NUSAP1 may associate with chromosomes and promote the organization of mitotic spindle microtubules around them. NUSAP1 plays a role in connecting apoptosis with cell migration and it is a potentially involved in morphogenesis in vertebrates (PMID:21203972). NUSAP1 is an immunogenic antigen in 65% of patients with AML following allogeneic HCT and suggests a tumor antigen role(PMID:20053754 ). NuSAP1 protein level is tightly regulated by the APC/C ubiquitin ligase complex and NuSAP induces mitotic arrest dependent of its microtubule affinity (PMID:17618083). NUSAP1 is overexpressed in pituitary adenomas.
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References
- Okamoto A, Higo M, Shiiba M. Down-Regulation of Nucleolar and Spindle-Associated Protein 1 (NUSAP1) Expression Suppresses Tumor and Cell Proliferation and Enhances Anti-Tumor Effect of Paclitaxel in Oral Squamous Cell Carcinoma. PloS one. 10(11):e0142252. 2015.
- Gulzar ZG, McKenney JK, Brooks JD. Increased expression of NuSAP in recurrent prostate cancer is mediated by E2F1. Oncogene. 32(1):70-7. 2013.
- Lee M, Marinoni I, Irmler M. Transcriptome analysis of MENX-associated rat pituitary adenomas identifies novel molecular mechanisms involved in the pathogenesis of human pituitary gonadotroph adenomas. Acta neuropathologica. 126(1):137-50. 2013.
- Song L, Craney A, Rape M. Microtubule-dependent regulation of mitotic protein degradation. Molecular cell. 53(2):179-92. 2014.
- Meyer HJ, Rape M. Enhanced protein degradation by branched ubiquitin chains. Cell. 157(4):910-21. 2014.
- Song L, Rape M. Regulated degradation of spindle assembly factors by the anaphase-promoting complex. Molecular cell. 38(3):369-82. 2010.
- Gordon CA, Gulzar ZG, Brooks JD. NUSAP1 expression is upregulated by loss of RB1 in prostate cancer cells. The Prostate. 75(5):517-26. 2015.
- Chen L, Yang L, Qiao F. High Levels of Nucleolar Spindle-Associated Protein and Reduced Levels of BRCA1 Expression Predict Poor Prognosis in Triple-Negative Breast Cancer. PloS one. 10(10):e0140572. 2015.
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