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Product Name
NUP85 antibody
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Description
NUP85 Rabbit Polyclonal antibody. Positive IHC detected in human stomach tissue, human liver tissue. Positive IF detected in hTERT-RPE1 cells. Positive WB detected in mouse lung tissue, HeLa cells, human brain tissue, mouse thymus tissue. Observed molecular weight by Western-blot: 70-75kd
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
85 kDa nucleoporin antibody; FLJ12549 antibody; FROUNT antibody; nucleoporin 85kDa antibody; Nucleoporin Nup75 antibody; Nucleoporin Nup85 antibody; Nup75 antibody; NUP85 antibody; PCNT1 antibody; Pericentrin 1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of NUP85 recombinant protein (Accession Number: NM_024844). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
mouse lung tissue were subjected to SDS PAGE followed by western blot with Catalog No:113383(NUP85 antibody) at dilution of 1:400
Immunohistochemical of paraffin-embedded human stomach using Catalog No:113383(NUP85 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human stomach using Catalog No:113383(NUP85 antibody) at dilution of 1:50 (under 40x lens)
IF result (Nucleus stain) of anti-NUP85 (Catalog No:113383; 1:50) hTERT-RPE1 cell (MeOH fixed) by Dr. Moshe Kim.
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Background
NUP85 represents nucleoporin 85kDa
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