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Product Name
NUMBL antibody
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Description
NUMBL Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IHC detected in human gliomas tissue, human lung cancer tissue. Positive WB detected in mouse brain tissue, HeLa cells, mouse liver tissue, mouse lung tissue, mouse spleen tissue. Positive IP detected in HeLa cells. Observed molecular weight by Western-blot: 65-70 kDa
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Tested applications
ELISA, IF, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CAG3A antibody; CTG3a antibody; NBL antibody; numb homolog (Drosophila) like antibody; Numb like protein antibody; NUMB R antibody; Numb related protein antibody; NUMBL antibody; NUMBLIKE antibody; NUMBR antibody; TNRC23 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of NUMBL recombinant protein (Accession Number: BC001794). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:113372(NUMBLIKE Antibody) at dilution of 1:2000
Immunohistochemistry of paraffin-embedded human gliomas tissue slide using Catalog No:113372(NUMBLIKE Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human gliomas tissue slide using Catalog No:113372(NUMBLIKE Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using NUMBL antibody Catalog No:113372 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
IP Result of anti-NUMBLIKE (IP:Catalog No:113372, 3ug; Detection:Catalog No:113372 1:1000) with HeLa cells lysate 1000ug.
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Background
Numbl (also known as numblike or Nbl), as a conserved homolog of Drosophila Numb, is specfically expressed in the brain. Numbl is a cytoplasm protein and has redundant functions in embryonic neurogenesis. It plays an important role in ependymal wall integrity and subventricular zone neuroblast survival. However, its expression and function in the central nervous system lesion are still unclear.
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References
- Vaira V, Faversani A, Martin NM. Regulation of lung cancer metastasis by Klf4-Numb-like signaling. Cancer research. 73(8):2695-705. 2013.
- Sinha-Hikim I, Cornford M, Gaytan H, Lee ML, Bhasin S. Effects of testosterone supplementation on skeletal muscle fiber hypertrophy and satellite cells in community-dwelling older men. The Journal of clinical endocrinology and metabolism. 91(8):3024-33. 2006.
- Rasin MR, Gazula VR, Breunig JJ. Numb and Numbl are required for maintenance of cadherin-based adhesion and polarity of neural progenitors. Nature neuroscience. 10(7):819-27. 2007.
- Moran TB, Goldberg LB, Serviss SL, Raetzman LT. Numb deletion in POMC-expressing cells impairs pituitary intermediate lobe cell adhesion, progenitor cell localization, and neuro-intermediate lobe boundary formation. Molecular endocrinology (Baltimore, Md.). 25(1):117-27. 2011.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"