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NRAS antibody

Cat.#: 113271

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Product Information

  • Product Name
    NRAS antibody
  • Documents
  • Description
    NRAS Rabbit Polyclonal antibody. Positive IF detected in HeLa cells. Positive IHC detected in human testis tissue, human pancreas tissue. Positive FC detected in HepG2 cells. Positive WB detected in Jurkat cells, A431 cells, HEK-293 cells. Observed molecular weight by Western-blot: 21 kDa
  • Tested applications
    ELISA, WB, IHC, FC, IF
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    ALPS4 antibody; GTPase NRas antibody; HRAS1 antibody; N ras antibody; NRAS antibody; NRAS1 antibody; Transforming protein N Ras antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of NRAS recombinant protein (Accession Number: NM_002524). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IHC: 1:20-1:200

    IF: 1:50-1:500

  • Validations

    Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:113271(NRAS antibody) at dilution of 1:500

    Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:113271(NRAS antibody) at dilution of 1:500

    Immunohistochemical of paraffin-embedded human testis using Catalog No:113271(NRAS antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemical of paraffin-embedded human testis using Catalog No:113271(NRAS antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemical of paraffin-embedded human testis using Catalog No:113271(NRAS antibody) at dilution of 1:50 (under 40x lens)

    Immunohistochemical of paraffin-embedded human testis using Catalog No:113271(NRAS antibody) at dilution of 1:50 (under 40x lens)

    Immunofluorescent analysis of (-20oc Ethanol) fixed HeLa cells using Catalog No:113271(NRAS Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

    Immunofluorescent analysis of (-20oc Ethanol) fixed HeLa cells using Catalog No:113271(NRAS Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

    1X10^6 HepG2 cells were stained with .2ug NRAS antibody (Catalog No:113271, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

    1X10^6 HepG2 cells were stained with .2ug NRAS antibody (Catalog No:113271, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

  • Background
    NRAS, also named as N-ras and NRAS1, is neuroblastoma RAS viral (v-ras) oncogene homolog from the mammalian ras gene family and it is a member of the small GTPase superfamily. RAS proteins are involved in signal transduction pathways, and bind GDP/GTP and possess intrinsic GTPase activity. It is mapped on chromosome 1, and it is activated in HL60, a promyelocytic leukemia line. Defects in NRAS are a cause of juvenile myelomonocytic leukemia (JMML).
  • References
    • Liu W, Ning R, Chen RN. Aspafilioside B induces G2/M cell cycle arrest and apoptosis by up-regulating H-Ras and N-Ras via ERK and p38 MAPK signaling pathways in human hepatoma HepG2 cells. Molecular carcinogenesis. 2015.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"