NR3C2 antibody

Cat.#: 113268

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Product Information

  • Product Name
    NR3C2 antibody
  • Documents
  • Description
    NR3C2 Rabbit Polyclonal antibody. Positive IP detected in HEK-293 cells. Positive WB detected in HEK-293 cells, HeLa cells. Positive IHC detected in human kidney tissue, human colon tissue, human skin tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 94-100kd
  • Tested applications
    ELISA, WB, IHC, IF, IP
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    MCR antibody; MineralOCorticoid receptor antibody; MLR antibody; NR3C2 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of NR3C2 recombinant protein (Accession Number: NM_001354819). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:200-1:2000

    IHC: 1:20-1:200

    IF: 1:10-1:100

  • Validations

    HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:113268(NR3C2 antibody) at dilution of 1:1000

    HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:113268(NR3C2 antibody) at dilution of 1:1000

    Immunohistochemical of paraffin-embedded human kidney using Catalog No:113268(NR3C2 antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemical of paraffin-embedded human kidney using Catalog No:113268(NR3C2 antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemical of paraffin-embedded human kidney using Catalog No:113268(NR3C2 antibody) at dilution of 1:50 (under 40x lens)

    Immunohistochemical of paraffin-embedded human kidney using Catalog No:113268(NR3C2 antibody) at dilution of 1:50 (under 40x lens)

    Immunofluorescent analysis of HepG2 cells, using NR3C2 antibody Catalog No:113268 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

    Immunofluorescent analysis of HepG2 cells, using NR3C2 antibody Catalog No:113268 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

    IP Result of anti-NR3C2 (IP:Catalog No:113268, 4ug; Detection:Catalog No:113268 1:500) with HEK-293 cells lysate 3200ug.

    IP Result of anti-NR3C2 (IP:Catalog No:113268, 4ug; Detection:Catalog No:113268 1:500) with HEK-293 cells lysate 3200ug.

  • Background
    Nuclear receptor subfamily 3 group C member 2 (NR3C2), also known as Mineralocorticoid receptor (MCR or MR), is a member of the steroid/thyroid /retinoic nuclear hormone receptor superfamily that has been shown to activate gene transcription in response to aldosterone binding. Regulation of the mineralocorticoid receptors occurs through either receptor down-regulation (negative autoregulation) or hormone-mediated upregulation (positive autoregulation). MCR association with HSP 90 appears to be required for hormone binding to MCR and subsequent MCR activation.This antibody is a rabbit polyclonal antibody, sepcifically reacts with the 107kd MCR protein.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"