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Product Name
NR2E3 antibody
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Description
NR2E3 Rabbit Polyclonal antibody. Positive WB detected in Y79 cells, HepG2 cells, mouse eye tissue. Positive FC detected in photoreceptor progenitor cells. Positive IF detected in photoreceptor progenitor cells. Positive IHC detected in human prostate cancer tissue. Observed molecular weight by Western-blot: 43-45 kDa
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Tested applications
ELISA, WB, IHC, IF, FC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ESCS antibody; NR2E3 antibody; PNR antibody; rd7 antibody; RNR antibody; RP37 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of NR2E3 recombinant protein (Accession Number: NM_001281446). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: N/A
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Validations
IF result of NR2E3 antibody (Catalog No:113266, 1:50) with human photoreceptor progenitors cells.
FC result of NR2E3 antibody (Catalog No:113266, 1:100) with human photoreceptor progenitors cells. Blue, isotype control; Black=passage 2; Red=passage 4.
Y79 cells were subjected to SDS PAGE followed by western blot with Catalog No:113266(NR2E3 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:113266(NR2E3 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:113266(NR2E3 antibody) at dilution of 1:100 (under 40x lens)
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Background
NR2E3, also known as PNR, encodes a retinal nuclear receptor that is a ligand-dependent transcription factor. This protein is part of a large family of nuclear receptor transcription factors involved in signaling pathways. NR2E3 influences the development of photoreceptors and their differentiation into rod and cone types, and acts as a ranscriptional factor that is an activator of rod development and repressor of cone development [PMID:20725840]. It binds the promoter region of a number of rod- and cone-specific genes, including rhodopsin, M- and S-opsin and rod-specific phosphodiesterase beta subunit. [PMID:15689355]
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References
- Cho KI, Haque M, Wang J. Distinct and atypical intrinsic and extrinsic cell death pathways between photoreceptor cell types upon specific ablation of Ranbp2 in cone photoreceptors. PLoS genetics. 9(6):e1003555. 2013.
- Cho KI, Patil H, Senda E. Differential loss of prolyl isomerase or chaperone activity of Ran-binding protein 2 (Ranbp2) unveils distinct physiological roles of its cyclophilin domain in proteostasis. The Journal of biological chemistry. 289(8):4600-25. 2014.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"