-
Product Name
NR1D1 antibody
- Documents
-
Description
NR1D1 Rabbit Polyclonal antibody. Positive IF detected in NIH/3T3 cells. Positive IP detected in A549 cells. Positive WB detected in NIH/3T3 cells, A549 cells, HeLa cells, MCF-7 cells. Observed molecular weight by Western-blot: 55-68 kDa
-
Tested applications
ELISA, WB, IP, IF
-
Species reactivity
Human, Mouse; other species not tested.
-
Alternative names
ear 1 antibody; EAR1 antibody; hRev antibody; NR1D1 antibody; Rev erbA alpha antibody; Reverb alpha antibody; THRA1 antibody; THRAL antibody; V erbA related protein 1 antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of NR1D1 recombinant protein (Accession Number: NM_021724). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:1000
IF: 1:50-1:500
-
Validations
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with Catalog No:113229(NR1D1 Antibody) at dilution of 1:1000
IP Result of anti-NR1D1 (IP:Catalog No:113229, 4ug; Detection:Catalog No:113229 1:300) with A549 cells lysate 1200ug.
Immunofluorescent analysis of (10% Formaldehyde) fixed NIH/3T3 cells using Catalog No:113229(NR1D1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
-
Background
NR1D1, also named as EAR1, THRAL, ear-1 and hRev, contains one nuclear receptor DNA-binding domain and belongs to the nuclear hormone receptor family. NR1 subfamily. This protein functions as a constitutive transcriptional repressor. It is a possible receptor for triiodothyronine. Rev-erba itself is activated by BMAL1-CLocK and thereby represents the link between the positive and negative loops of the circadian clock. This is a rabbit polyclonal antibody raised against the N terminus of human NR1D1.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"