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Product Name
NQO2 antibody
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Description
NQO2 Rabbit Polyclonal antibody. Positive IHC detected in human brain tissue. Positive IF detected in Hela cells. Positive WB detected in HeLa cells, human liver tissue. Positive IP detected in HeLa cells. Observed molecular weight by Western-blot: 26kd
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
DHQV antibody; DIA6 antibody; NQO2 antibody; NRH dehydrogenase [quinone] 2 antibody; NRH:quinone oxidoreductase 2 antibody; QR2 antibody; Quinone reductase 2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of NQO2 recombinant protein (Accession Number: NM_001318940). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:113228(NQO2 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human brain using Catalog No:113228(NQO2 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human brain using Catalog No:113228(NQO2 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of Hela cells, using NQO2 antibody Catalog No:113228 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-NQO2 (IP:Catalog No:113228, 3ug; Detection:Catalog No:113228 1:500) with HeLa cells lysate 1200ug.
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Background
NQO2, also named as QR2 and NMOR2, belongs to the NAD(P)H dehydrogenase (quinone) family. It serves as a quinone reductase in connection with conjugation reactions of hydroquinones involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis. The cytosolic quinone oxidoreductases NQO1 and NQO2 protect cells against oxidative stress by detoxifying quinones and preventing redox cycling. NQO1 and NQO2 are important endogenous factors in regulation of immune response and autoimmunity.
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References
- Zhang XW, Wang Y, Cai JB. Downregulation of quinone reductase 2 attenuates vascular smooth muscle cells proliferation and neointimal formation in balloon injured rat carotid artery. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology. 29(3-4):453-62. 2012.
- He T, Hatem E, Vernis L, Lei M, Huang ME. PRX1 knockdown potentiates vitamin K3 toxicity in cancer cells: a potential new therapeutic perspective for an old drug. Journal of experimental & clinical cancer research : CR. 34:152. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"