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Product Name
NPM3 antibody
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Description
NPM3 Rabbit Polyclonal antibody. Positive IHC detected in human lymphoma tissue. Positive IF detected in HeLa cells. Positive WB detected in HeLa cells. Positive IP detected in HeLa cells. Observed molecular weight by Western-blot: 25 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
NPM3 antibody; nucleophosmin/nucleoplasmin antibody; Nucleoplasmin 3 antibody; PORMIN antibody; TMEM123 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of NPM3 recombinant protein (Accession Number: NM_006993). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:113219(NPM3 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human lymphoma using Catalog No:113219(NPM3 antibody) at dilution of 1:50 (under 10x lens)
IP Result of anti-NPM3 (IP:Catalog No:113219, 4ug; Detection:Catalog No:113219 1:500) with HeLa cells lysate 3400ug.
Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using Catalog No:113219(NPM3 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
NPM3 represents nucleophosmin/nucleoplasmin, 3
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