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Product Name
NPHS2 antibody
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Description
NPHS2 Rabbit Polyclonal antibody. Positive IF detected in zebrafish embryos. Positive WB detected in mouse colon tissue, Transfected HEK-293 cells. Observed molecular weight by Western-blot: 34kd
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Tested applications
ELISA, WB, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
NPHS2 antibody; PDCN antibody; PodOCin antibody; SRN1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of NPHS2 recombinant protein (Accession Number: NM_001297575). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IF: 1:20-1:200
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Validations
The podocin antibody Catalog No:113216 recognized the zebrafish pronephric glomeruli in zebrafish embryos(72 hour-old fish). IF result from Dr. Weibin Zhou.
mouse colon tissue were subjected to SDS PAGE followed by western blot with Catalog No:113216(NPHS2 antibody) at dilution of 1:400
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Background
NPHS2 represents nephrosis 2, idiopathic, steroid-resistant (podocin)
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References
- Wang Z, Liu J, Sun W. Effects of asiaticoside on levels of podocyte cytoskeletal proteins and renal slit diaphragm proteins in adriamycin-induced rat nephropathy. Life sciences. 93(8):352-8. 2013.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"