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Product Name
NOSTRIN antibody
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Description
NOSTRIN Rabbit Polyclonal antibody. Positive IHC detected in human heart tissue. Positive IF detected in HepG2 cells. Positive WB detected in mouse heart tissue, human heart tissue, human placenta tissue, K-562 cells, mouse lung tissue. Positive IP detected in human placenta tissue. Observed molecular weight by Western-blot: 58 kDa
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Tested applications
ELISA, IP, IF, WB, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
BM247 homolog antibody; DaIP2 antibody; eNOS trafficking inducer antibody; NOSTRIN antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of NOSTRIN recombinant protein (Accession Number: NM_052946). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:50-1:500
IF: 1:20-1:200
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Validations
Immunohistochemistry of paraffin-embedded human heart tissue slide using Catalog No:113306(NOSTRIN Antibody) at dilution of 1:200 (under 10x lens).
Immunohistochemistry of paraffin-embedded human heart tissue slide using Catalog No:113306(NOSTRIN Antibody) at dilution of 1:200 (under 40x lens).
mouse heart tissue were subjected to SDS PAGE followed by western blot with Catalog No:113306(NOSTRIN Antibody) at dilution of 1:600
IP Result of anti-NOSTRIN (IP:Catalog No:113306, 4ug; Detection:Catalog No:113306 1:500) with human placenta tissue lysate 1600ug.
Immunofluorescent analysis of HepG2 cells using Catalog No:113306(NOSTRIN Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
NOSTRIN represents nitric oxide synthase trafficker
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"