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Product Name
NMT1 antibody
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Description
NMT1 Rabbit Polyclonal antibody. Positive WB detected in SKOV-3 cells, HeLa cells, human kidney tissue, L02 cells, mouse pancreas tissue, PC-3 cells. Positive IP detected in HeLa cells. Positive IHC detected in human gliomas tissue, human heart tissue, human kidney tissue. Positive IF detected in MCF-7 cells. Observed molecular weight by Western-blot: 49-68 kDa
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Tested applications
ELISA, IHC, IF, WB, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
N myristoyltransferase 1 antibody; NMT1 antibody; Type I N myristoyltransferase antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of NMT1 recombinant protein (Accession Number: NM_021079). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
SKOV-3 cells were subjected to SDS PAGE followed by western blot with Catalog No:113255(NMT1 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human gliomas using Catalog No:113255(NMT1 antibody) at dilution of 1:50 (under 10x lens)
Immunofluorescent analysis of MCF-7 cells, using NMT1 antibody Catalog No:113255 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-NMT1 (IP:Catalog No:113255, 4ug; Detection:Catalog No:113255 1:500) with HeLa cells lysate 2000ug.
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Background
NMT1 is a N-myristoyltransferase responsible for the transfer of myristate from CoA to an amino-terminal glycine of many eukaryotic proteins, which facilitates the targeting of proteins to membrane surfaces and is essential for viability of the organism. Insertional mutagenesis of the Nmt1 gene in Saccharomyces cerevisiae causes recessive lethality. Humans and mice possess two distinct but structurally similar enzymes, NMT1 and NMT2, ubiquitously expressed in most human and mouse tissues. Western analysis revealed that there are 4 isoforms of NMT1 with apparent molecular masses ranging from 49 to 68 kD. In cell fractionation studies, the 68-kD NMT1 isoform and NMT2 were present in both membrane and cytoplasmic fractions, while the smaller NMT1 isoforms were predominantly cytoplasmic.
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References
- Perinpanayagam MA, Beauchamp E, Martin DD, Sim JY, Yap MC, Berthiaume LG. Regulation of co- and post-translational myristoylation of proteins during apoptosis: interplay of N-myristoyltransferases and caspases. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 27(2):811-21. 2013.
- Dudek E, Millott R, Liu WX, Beauchamp E, Berthiaume LG, Michalak M. N-myristoyltransferase 1 interacts with calnexin at the endoplasmic reticulum. Biochemical and biophysical research communications. 468(4):889-93. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"