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Product Name
NME2 antibody
- Documents
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Description
NME2 Rabbit Polyclonal antibody. Positive WB detected in mouse kidney tissue, Jurkat cells, mouse liver tissue. Positive IHC detected in human kidney tissue, human heart tissue. Observed molecular weight by Western-blot: 17kd
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
NDK B antibody; NDP kinase B antibody; NDPK B antibody; NDPKB antibody; NME2 antibody; puf antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of NME2 recombinant protein (Accession Number: NM_001018137). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
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Validations
mouse kidney tissue were subjected to SDS PAGE followed by western blot with Catalog No:113247(NME2 antibody) at dilution of 1:200
Immunohistochemical of paraffin-embedded human kidney using Catalog No:113247(NME2 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human kidney using Catalog No:113247(NME2 antibody) at dilution of 1:50 (under 40x lens)
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Background
NME2(non-metastatic cells 2, protein) is also named as NDKB(Nucleoside diphosphate kinase B), NM23B and belongs to the NDK family. It is involved in the phosphorylation of nucleoside diphosphates and interacts with membrane receptor and constituting a novel molecular regulatory mechanism of GPCR endocytosis. This protein has 2 isoforms produced by alternative splicing.
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References
- Lu Y, Cai G, Cui S. FHL2-driven molecular network mediated Septin2 knockdown inducing apoptosis in mesangial cell. Proteomics. 14(21-22):2485-97. 2014.
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