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Product Name
NKIRAS2 antibody
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Description
NKIRAS2 Rabbit Polyclonal antibody. Positive IF detected in A431 cells. Positive WB detected in HEK-293 cells, HepG2 cells. Observed molecular weight by Western-blot: 22 kDa
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Tested applications
ELISA, WB, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
DKFZp434N1526 antibody; Kappa B Ras protein 2 antibody; kappaB Ras2 antibody; KBRAS2 antibody; NKIRAS2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of NKIRAS2 recombinant protein (Accession Number: XM_047435822). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IF: 1:20-1:200
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:113199(NKIRAS2 Antibody) at dilution of 1:600
Immunofluorescent analysis of A431 cells using Catalog No:113199(NKIRAS2 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
I-Kappa-B-interacting Ras-like protein 2 (KBRAS2, MGC:4553), interact with the PEST domains of IkappaBalpha and IkappaBbeta [inhibitors of the transcription factor nuclear factor kappa B (NF-kappaB)] and decrease their rate of degradation. KBRAS2 differs from other Ras proteins in containing Ala and Leu residues at positions 12 and 61 respectively, which are similar to those present in the oncogenic forms of Ras.
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