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Product Name
NIP7 antibody
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Description
NIP7 Rabbit Polyclonal antibody. Positive WB detected in mouse liver tissue, HeLa cells, HepG2 cells. Positive IP detected in mouse liver tissue. Observed molecular weight by Western-blot: 20,15kd
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Tested applications
ELISA, WB, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CGI 37 antibody; FLJ10296 antibody; HSPC031 antibody; HSPC180 antibody; KD93 antibody; NIP7 antibody; OK/SW cl.76 antibody; OK/SW cl.78 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of NIP7 recombinant protein (Accession Number: NM_016101). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
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Validations
mouse liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:113187(NIP7 antibody) at dilution of 1:500
IP Result of anti-NIP7 (IP:Catalog No:113187, 3ug; Detection:Catalog No:113187 1:500) with mouse liver tissue lysate 4000ug.
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Background
Nip7 was initially identified in yeast as required for processing of the 27S pre-rRNA to form the mature 25S and 5.8S rRNAs (PMID: 9891085). It localizes to the nucleolus but was also found to sediment in the region of free 60S subunits in sucrose density gradients (PMID: 9891085). Experimental evidence suggests that the P. abyssi Nip7 may be an exosome regulatory factor. It binds preferentially to U- and AU-rich RNAs and strongly inhibits the exosome due to its association with both the exosome complex and the substrate RNA.
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