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Product Name
NHP2 antibody
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Description
NHP2 Rabbit Polyclonal antibody. Positive IHC detected in human prostate cancer tissue. Positive IF detected in HepG2 cells. Positive WB detected in A375 cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells. Positive IP detected in HepG2 cells. Observed molecular weight by Western-blot: 17-20kd
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
NHP2 antibody; NHP2P antibody; NOLA2 antibody; snoRNP protein NHP2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of NHP2 recombinant protein (Accession Number: NM_017838). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
A375 cells were subjected to SDS PAGE followed by western blot with Catalog No:113179(NHP2 antibody) at dilution of 1:300
Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:113179(NHP2 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using NHP2 antibody Catalog No:113179 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-NHP2 (IP:Catalog No:113179, 4ug; Detection:Catalog No:113179 1:500) with HepG2 cells lysate 3600ug.
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Background
NHP2 belongs to the H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA. It is required for ribosome biogenesis and telomere maintenance, and for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transcriptase (TERT) holoenzyme.
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References
- Kim MS, Kim SS, Yoo NJ, Lee SH. Expressional analysis of NOLA1, NOLA2, NOLA3 and DKC1, the core proteins in H/ACA riboproteins, in gastric and colorectal cancers. Pathology. 44(6):576-7. 2012.
- Lin P, Mobasher ME, Hakakian Y. Differential requirements for H/ACA ribonucleoprotein components in cell proliferation and response to DNA damage. Histochemistry and cell biology. 144(6):543-58. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"