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Product Name
NFIL3 antibody
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Description
NFIL3 Rabbit Polyclonal antibody. Positive WB detected in HepG2 cells, HEK-293 cells, HeLa cells, human testis tissue, K-562 cells, mouse heart tissue, mouse lung tissue. Positive IP detected in HeLa cells. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 63 kDa,50-51 kDa
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Tested applications
ELISA, WB, IP, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
E4 promoter binding protein 4 antibody; E4BP4 antibody; IL3BP1 antibody; NF IL3A antibody; NFIL3 antibody; NFIL3A antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of NFIL3 recombinant protein (Accession Number: XM_047423422). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:500-1:5000
IF: 1:50-1:500
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:113152(NFIL3 antibody) at dilution of 1:1000
IP Result of anti-NFIL3 (IP:Catalog No:113152, 4ug; Detection:Catalog No:113152 1:1000) with HeLa cells lysate 1600ug.
Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using Catalog No:113152(NFIL3 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
NFIL3, also named as E4BP4 and IL3BP1, belongs to the bZIP family and NFIL3 subfamily. It represses transcription from promoters with activating transcription factor (ATF) sites. NFIL3 activates transcription from the interleukin-3 promoter in T-cells. It competes for the same consensus-binding site with PAR DNA-binding factors (DBP, HLF and TEF). It is a component of the circadian clock that acts as a negative regulator for the circadian expression of PER2 oscillation in the cell-autonomous core clock. NFIL3 protects pro-B cells from programmed cell death. It is a key regulator of TH2 responses(PMID:21499227).
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