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Product Name
NF2 antibody
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Description
NF2 Rabbit Polyclonal antibody. Positive IHC detected in human meningioma tissue, mouse brain tissue. Positive IP detected in HeLa cells. Positive WB detected in HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, MCF7 cells, NIH/3T3 cells, SH-SY5Y cells. Observed molecular weight by Western-blot: 66-70 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ACN antibody; BANF antibody; Merlin antibody; Neurofibromin 2 antibody; neurofibromin 2 (merlin) antibody; NF2 antibody; SCH antibody; Schwannomerlin antibody; Schwannomin antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of NF2 recombinant protein (Accession Number: NM_000268). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:113136(NF2 antibody) at dilution of 1:1000
IP Result of anti-NF2 (IP:Catalog No:113136, 4ug; Detection:Catalog No:113136 1:600) with HeLa cells lysate 2400ug.
Immunohistochemistry of paraffin-embedded human meningioma tissue slide using Catalog No:113136(NF2 Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human meningioma tissue slide using Catalog No:113136(NF2 Antibody) at dilution of 1:50 (under 40x lens)
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Background
NF2 represents neurofibromin 2 (merlin)
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References
- Gao Y, Wang Y, Feng J. A hairpin within YAP mRNA 3'UTR functions in regulation at post-transcription level. Biochemical and biophysical research communications. 459(2):306-12. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"