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Product Name
Nestin antibody
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Description
Nestin Mouse Monoclonal antibody. Positive IF detected in U-251 cells. Positive IHC detected in human gliomas tissue. Positive WB detected in Neuro-2a cells, HEK-293 cells, rat brain tissue. Observed molecular weight by Western-blot: 150-170 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human, Mouse, Rat; other species not tested.
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Alternative names
NES antibody; Nestin antibody
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Isotype
Mouse IgG2b
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_006617). Purification method: Protein A purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
Neuro-2a cells were subjected to SDS PAGE followed by western blot with Catalog No:107329(Nestin Antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human gliomas tissue slide using (Nestin Antibody) at dilution of 1:200 (under 10x lens).
Immunohistochemistry of paraffin-embedded human gliomas tissue slide using (Nestin Antibody) at dilution of 1:200 (under 40x lens).
Immunofluorescent analysis of (10% Formaldehyde) fixed U-251 cells using Catalog No:107329(Nestin Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L)
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Background
Nestin, also named as NES and Nbla00170, is a class VI intermediate filament protein expressed in stem cells of the central nervous system (CNS) but not in mature CNS cells. Its expression is used extensively as a marker for neural lineage cells derived from human embryonic stem (hES) and induced pluripotent stem (iPS) cells. Nestin is also expressed in non-neural stem cell populations such as pancreatic islet progenitors and hematopoietic progenitors.
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