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Product Name
NELFE antibody
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Description
NELFE Rabbit Polyclonal antibody. Positive IP detected in mouse brain tissue. Positive WB detected in HEK-293 cells, HeLa cells, mouse brain tissue, mouse eye tissue, mouse placenta tissue, rat brain tissue. Positive IHC detected in human prostate cancer tissue. Observed molecular weight by Western-blot: 44kd
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
D6S45 antibody; Negative elongation factor E antibody; NELF E antibody; NELFE antibody; RD RNA binding protein antibody; RDBP antibody; RDP antibody; RNA binding protein RD antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of NELFE recombinant protein (Accession Number: BC011600). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:113111(RDBP antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human prostate cancer using Catalog No:113111(RDBP antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-RDBP (IP:Catalog No:113111, 4ug; Detection:Catalog No:113111 1:800) with mouse brain tissue lysate 4680ug.
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Background
RDBP, also named as NELF-E and RD, belongs to the RRM NELF-E family. It is an essential component of the NELF complex which is a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, a complex acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. RDBP was recently found implicated in RNA polymerase II pausing by association with Hsp90. Catalog# 10705-1-AP is a widely used rabbit polyclonal antibdoy obtained by immunizing animals with full-length RDBP of human origin.
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References
- Iida M, Iizuka N, Tsunedomi R. Overexpression of the RD RNA binding protein in hepatitis C virus-related hepatocellular carcinoma. Oncology reports. 28(2):728-34. 2012.
- Gupte R, Muse GW, Chinenov Y, Adelman K, Rogatsky I. Glucocorticoid receptor represses proinflammatory genes at distinct steps of the transcription cycle. Proceedings of the National Academy of Sciences of the United States of America. 110(36):14616-21. 2013.
- Bastide MF, Bido S, Duteil N, Bézard E. Striatal NELF-mediated RNA polymerase II stalling controls l-dopa induced dyskinesia. Neurobiology of disease. 85:93-8. 2016.
- Adelman K, Kennedy MA, Nechaev S. Immediate mediators of the inflammatory response are poised for gene activation through RNA polymerase II stalling. Proceedings of the National Academy of Sciences of the United States of America. 106(43):18207-12. 2009.
- Sawarkar R, Sievers C, Paro R. Hsp90 globally targets paused RNA polymerase to regulate gene expression in response to environmental stimuli. Cell. 149(4):807-18. 2012.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"