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Product Name
NELFB antibody
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Description
NELFB Rabbit Polyclonal antibody. Positive IF detected in BxPC-3 cells. Positive WB detected in BxPC-3 cells, HepG2 cells. Positive IP detected in mouse brain tissue. Observed molecular weight by Western-blot: 66 kDa
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Tested applications
ELISA, IP, IF, WB
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
COBRA1 antibody; cofactor of BRCA1 antibody; DKFZp586B0519 antibody; KIAA1182 antibody; Negative elongation factor B antibody; NELF B antibody; NELFB antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of NELFB recombinant protein (Accession Number: BC011892). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IF: 1:10-1:100
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Validations
BxPC-3 cells were subjected to SDS PAGE followed by western blot with Catalog No:113110(NELF-B Antibody) at dilution of 1:1000
IP Result of anti-NELF-B (IP:Catalog No:113110, 3ug; Detection:Catalog No:113110 1:500) with mouse brain tissue lysate 6000ug.
Immunofluorescent analysis of BxPC-3 cells using Catalog No:113110(NELF-B Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
Cofactor of BRCA1 (COBRA1) was first identified as a BRCA1-interacting protein and subsequently found to be the B subunit of the negative elongation factor complex (NELF-B). Cobra1 is essential for early embryogenesis (PMID: 19340312). The MW of this protein is 66 kDa, and this antibody specially recognises the 66 kDa protein.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"