NEK9 antibody

Cat.#: 113107

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Product Information

  • Product Name
    NEK9 antibody
  • Documents
  • Description
    NEK9 Rabbit Polyclonal antibody. Positive IF detected in COS-7 cells.
  • Tested applications
    ELISA, IF
  • Species reactivity
    Human; other species not tested.
  • Alternative names
    DKFZp434D0935 antibody; KIAA1995 antibody; Nek8 antibody; NEK9 antibody; NERCC antibody; NERCC1 antibody; Nercc1 kinase antibody; NimA related kinase 8 antibody; NimA related protein kinase 9 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of NEK9 recombinant protein (Accession Number: BC009336). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    IF: 1:20-1:200

  • Validations

    Immunofluorescent analysis of (10% Formaldehyde) fixed COS-7 cells using Catalog No:113107(NEK9 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

    Immunofluorescent analysis of (10% Formaldehyde) fixed COS-7 cells using Catalog No:113107(NEK9 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

  • Background
    NEK9 is also named as KIAA1995, NEK8, NERCC and belongs to the NEK Ser/Thr protein kinase family. It is independently identified as a Nek6- and Ran GTPase- binding protein under a different name, Nercc1 (PMID:14660563). Nek9, together with the highly similar Nek6 and Nek7, form a signaling module that is activated during mitosis and involved in the regulation of the mitotic spindle. Confocal microscopy identified that Nek9 was localized to the spindle poles at the metaphase stages and associated with the midbody at anaphase or telophase stage in both meiotic oocytes and the first mitotic embyros (PMID: 23159858). The endogenous Nek9 (with predicted molecular mass of 120 kDa) has an apparent molecular mass of 600 kDa, also compatible with a tetramer (PMID:21454704).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"