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Product Name
NEK9 antibody
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Description
NEK9 Rabbit Polyclonal antibody. Positive IP detected in HeLa cells. Positive WB detected in HeLa cells, A549 cells, human placenta tissue. Positive FC detected in HepG2 cells. Positive IHC detected in human lymphoma tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 120kd
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Tested applications
ELISA, WB, IHC, IF, FC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
DKFZp434D0935 antibody; KIAA1995 antibody; Nek8 antibody; NEK9 antibody; NERCC antibody; NERCC1 antibody; Nercc1 kinase antibody; NimA related kinase 8 antibody; NimA related protein kinase 9 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of NEK9 recombinant protein (Accession Number: BC009336). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:113106(NEK9 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human lymphoma using Catalog No:113106(NEK9 antibody) at dilution of 1:50 (under 10x lens)
Immunofluorescent analysis of HepG2 cells, using NEK9 antibody Catalog No:113106 at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).
IP Result of anti-NEK9 (IP:Catalog No:113106, 5ug; Detection:Catalog No:113106 1:500) with HeLa cells lysate 2000ug.
1X10^6 HepG2 cells were stained with .2ug NEK9 antibody (Catalog No:113106, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
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Background
NEK9 is also named as KIAA1995, NEK8, NERCC and belongs to the NEK Ser/Thr protein kinase family. It is originally identified as αβ-casein kinase that associates with a putative substrate Bicd2. It is also independently identified as a Nek6- and Ran GTPase-binding protein under a different name, Nercc1(PMID:14660563). Nek9, together with the highly similar (80% identical) Nek6 and Nek7, form a signaling module that is activated during mitosis and involved in the regulation of the mitotic spindle. The endogenous Nek9 (with predicted molecular mass of 120 kDa) has an apparent molecular mass of 600 kDa, also compatible with a tetramer(PMID:21454704).
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