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Product Name
NDUFV1 antibody
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Description
NDUFV1 Rabbit Polyclonal antibody. Positive IP detected in A431 cells. Positive WB detected in A431 cells, human brain tissue. Positive IHC detected in human lung cancer tissue. Observed molecular weight by Western-blot: 51 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CI 51kD antibody; Complex I 51kD antibody; NDUFV1 antibody; UQOR1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of NDUFV1 recombinant protein (Accession Number: NM_007103). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
A431 cells were subjected to SDS PAGE followed by western blot with Catalog No:113086(NDUFV1 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human lung cancer using Catalog No:113086(NDUFV1 antibody) at dilution of 1:50 (under 10x lens)
IP Result of anti-NDUFV1 (IP:Catalog No:113086, 4ug; Detection:Catalog No:113086 1:500) with A431 cells lysate 2000ug.
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Background
DUFV1(NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial) is also named as UQOR1,Complex I-51kD and belongs to the complex I 51 kDa subunit family. It is the core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. It has 2 isoforms produced by alternative splicing. Defects in NDUFV1 are a cause of Leigh syndrome (LS) and mitochondrial complex I deficiency (MT-C1D).
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References
- Kelly-Aubert M, Trudel S, Fritsch J. GSH monoethyl ester rescues mitochondrial defects in cystic fibrosis models. Human molecular genetics. 20(14):2745-59. 2011.
- Paterson AW, Curtis JC, Macleod NK. Complex I specific increase in superoxide formation and respiration rate by PrP-null mouse brain mitochondria. Journal of neurochemistry. 105(1):177-91. 2008.
- Lopes Costa A, Le Bachelier C, Mathieu L. Beneficial effects of resveratrol on respiratory chain defects in patients' fibroblasts involve estrogen receptor and estrogen-related receptor alpha signaling. Human molecular genetics. 23(8):2106-19. 2014.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"