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Product Name
NDUFS1 antibody
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Description
NDUFS1 Rabbit Polyclonal antibody. Positive WB detected in human kidney tissue, A549 cells. Positive IP detected in mouse lung tissue. Positive IHC detected in human gliomas tissue. Positive IF detected in Hela cells. Observed molecular weight by Western-blot: 79 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CI 75Kd antibody; Complex I 75kD antibody; NDUFS1 antibody; PRO1304 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of NDUFS1 recombinant protein (Accession Number: NM_005006). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
human kidney tissue were subjected to SDS PAGE followed by western blot with Catalog No:113081(NDUFS1 antibody) at dilution of 1:400
Immunohistochemical of paraffin-embedded human gliomas using Catalog No:113081(NDUFS1 antibody) at dilution of 1:50 (under 10x lens)
Immunofluorescent analysis of Hela cells, using NDUFS1 antibody Catalog No:113081 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
IP Result of anti-NDUFS1 (IP:Catalog No:113081, 4ug; Detection:Catalog No:113081 1:500) with mouse lung tissue lysate 2800ug.
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Background
The multisubunit NADH:ubiquinone oxidoreductase (75 kDa subunit, mitochondrial)(NDUFS1) is the first enzyme complex in the electron transport chain of mitochondria. It is also named as Complex I-75kD. By use of chaotropic agents, complex I can be fragmented into 3 different fractions: a flavoprotein fraction, an iron-sulfur protein (IP) fraction, and a hydrophobic protein (HP) fraction. NDUFS1 is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme.
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References
- Shen SM, Yu Y, Wu ZX, Zheng Y, Chen GQ, Wang LS. Apoptosis-inducing factor is a target gene of C/EBPα and participates in adipocyte differentiation. FEBS letters. 585(14):2307-12. 2011.
- Alvarez-Fischer D, Fuchs J, Castagner F. Engrailed protects mouse midbrain dopaminergic neurons against mitochondrial complex I insults. Nature neuroscience. 14(10):1260-6. 2011.
- Chouchani ET, Methner C, Nadtochiy SM. Cardioprotection by S-nitrosation of a cysteine switch on mitochondrial complex I. Nature medicine. 19(6):753-9. 2013.
- Andrews B, Carroll J, Ding S, Fearnley IM, Walker JE. Assembly factors for the membrane arm of human complex I. Proceedings of the National Academy of Sciences of the United States of America. 110(47):18934-9. 2013.
- Sun S, Han Y, Liu J. Trichostatin A targets the mitochondrial respiratory chain, increasing mitochondrial reactive oxygen species production to trigger apoptosis in human breast cancer cells. PloS one. 9(3):e91610. 2014.
- Shen SM, Guo M, Xiong Z. AIF inhibits tumor metastasis by protecting PTEN from oxidation. EMBO reports. 16(11):1563-80. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"