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Product Name
NDUFB2 antibody
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Description
NDUFB2 Rabbit Polyclonal antibody. Positive IHC detected in human brain tissue, human heart tissue, human kidney tissue, human liver tissue, human ovary tissue, human placenta tissue, human skin tissue, human spleen tissue, human testis tissue. Positive WB detected in human heart tissue. Observed molecular weight by Western-blot: 12kd
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
AGGG antibody; CI AGGG antibody; Complex I AGGG antibody; NDUFB2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of NDUFB2 recombinant protein (Accession Number: BC063026). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
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Validations
human heart tissue were subjected to SDS PAGE followed by western blot with Catalog No:113072(NDUFB2 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human brain using Catalog No:113072(NDUFB2 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human brain using Catalog No:113072(NDUFB2 antibody) at dilution of 1:100 (under 40x lens)
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Background
The multisubunit NADH:ubiquinone oxidoreductase (complex I) is the first enzyme complex in the electron transport chain of mitochondria. NDUFB2, also named as CI-AGGG, is a nuclear encoded subunit located in the hydrophobic protein fraction of complex I(PMID:9878551). It may have a role in the regulation of molecular changes during the global cardiac ischemia/reperfusion injury during cardiopulmonary bypass (CPB). The full length 12 kDa protein has a transit peptide with 33 amino acids.
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