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Product Name
NDUFB11 antibody
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Description
NDUFB11 Rabbit Polyclonal antibody. Positive IHC detected in human kidney tissue, human heart tissue, human liver tissue, human ovary tissue, human skin tissue, human spleen tissue, human testis tissue. Positive IF detected in HepG2 cells. Positive WB detected in HepG2 cells, mouse pancreas tissue, mouse skeletal muscle tissue, PC-3 cells, rat skeletal muscle tissue. Observed molecular weight by Western-blot: 18-20 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human, Mouse, Rat; other species not tested.
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Alternative names
CI ESSS antibody; Complex I ESSS antibody; ESSS antibody; FLJ20494 antibody; NDUFB11 antibody; Neuronal protein 17.3 antibody; P17.3 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of NDUFB11 recombinant protein (Accession Number: NM_019056). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:113071(NDUFB11 antibody) at dilution of 1:800
Immunohistochemical of paraffin-embedded human kidney using Catalog No:113071(NDUFB11 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human kidney using Catalog No:113071(NDUFB11 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of HepG2 cells using Catalog No:113071(NDUFB11 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
NDUFB11(NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial) is also named as neuronal protein 17.3(Np17.3) and belongs to the complex I NDUFB11 subunit family. This protein is involved in the transfer of electrons from NADH to the respiratory chain and play a role in the growth, maintenance, and survival of neurons. NDUFB11 has 2 isoforms produced by alternative splicing.
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References
- van Rahden VA, Fernandez-Vizarra E, Alawi M. Mutations in NDUFB11, encoding a complex I component of the mitochondrial respiratory chain, cause microphthalmia with linear skin defects syndrome. American journal of human genetics. 96(4):640-50. 2015.
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