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Product Name
NCAPH antibody
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Description
NCAPH Rabbit Polyclonal antibody. Positive IF detected in HepG2. Positive IHC detected in human colon cancer. Positive WB detected in HeLa cells, A375 cells. Positive IP detected in HeLa cells. Observed molecular weight by Western-blot: 83kd
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Barren homolog protein 1 antibody; BRRN antibody; BRRN1 antibody; CAP H antibody; CAPH antibody; Condensin complex subunit 2 antibody; HCAP H antibody; KIAA0074 antibody; NCAPH antibody; XCAP H homolog antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of NCAPH recombinant protein (Accession Number: NM_015341). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:113032(NCAPH antibody) at dilution of 1:500
IP Result of anti-NCAPH (IP:Catalog No:113032, 3ug; Detection:Catalog No:113032 1:800) with HeLa cells lysate 3000ug.
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:113032(NCAPH antibody) at dilution of 1:50 (under 10x lens)
Immunofluorescent analysis of HepG2 cells, using NCAPH antibody Catalog No:113032 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
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Background
NCAPH/BRRN gene encodes a member of the BARR gene family and a regulatory subunit of the condensin complex, which contains the SMC2 and SMC4 heterodimer, and three non SMC subunits that probably regulate the complex. This complex is required for the conversion of interphase chromatin into condensed chromosomes. The protein hCAP-H, encoded by NCAPH gene, is associated with mitotic chromosomes, except during the early phase of chromosome condensation. During interphase hCAP-H, -C, and -E, have distinct punctate nucleolar localization, suggesting that condensin may associate with and modulate the conformation and function of rDNA (PMID: 11694586).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"