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Product Name
NCAPD2 antibody
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Description
NCAPD2 Rabbit Polyclonal antibody. Positive IP detected in HEK-293 cells. Positive WB detected in HEK-293 cells, HeLa cells, K-562 cells. Positive IF detected in Hela cells. Observed molecular weight by Western-blot: 157 kDa
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Tested applications
ELISA, IF, WB, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CAP D2 antibody; CAPD2 antibody; CNAP1 antibody; Condensin complex subunit 1 antibody; hCAP D2 antibody; KIAA0159 antibody; NCAPD2 antibody; XCAP D2 homolog antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of NCAPD2 recombinant protein (Accession Number: NM_014865). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IF: 1:20-1:200
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:113029(NCAPD2 Antibody) at dilution of 1:600
Immunofluorescent analysis of Hela cells, using NCAPD2 antibody Catalog No:113029 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
IP Result of anti-NCAPD2 (IP:Catalog No:113029, 4ug; Detection:Catalog No:113029 1:600) with HEK-293 cells lysate 2800ug.
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Background
NCAPD2 is a regulatory subunit of the condensin complex, which is required for conversion of interphase chromatin into mitotic-like condense chromosomes. Condensin complexes are heteropentamers comprised of two SMC (structural maintenance of chromosomes) subunits and three non-SMC subunits. The C-terminus of NCAPD2 interacts with Histones H1 and H3 through their histone tails. A loss of NCAPD2 can lead to the disorganization of chromatid axes, misalignment of sister chromatids during metaphase and delayed entry into anaphase.
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