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Product Name
NAPB antibody
- Documents
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Description
NAPB Rabbit Polyclonal antibody. Positive WB detected in mouse brain tissue. Positive IP detected in mouse brain tissue. Positive IF detected in HepG2 cells. Positive IHC detected in human gliomas tissue. Observed molecular weight by Western-blot: 35-38 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
beta SNAP antibody; NAPB antibody; SNAP BETA antibody; SNAPB antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of NAPB recombinant protein (Accession Number: NM_022080). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:112947(NAPB antibody) at dilution of 1:1200
Immunohistochemical of paraffin-embedded human gliomas using Catalog No:112947(NAPB antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human gliomas using Catalog No:112947(NAPB antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using NAPB antibody Catalog No:112947 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
IP Result of anti-NAPB (IP:Catalog No:112947, 4ug; Detection:Catalog No:112947 1:1000) with mouse brain tissue lysate 4000ug.
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Background
NAPB represents N-ethylmaleimide-sensitive factor attachment protein, beta
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