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Product Name
N4BP3 antibody
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Description
N4BP3 Rabbit Polyclonal antibody. Positive IHC detected in human kidney tissue, human cervical cancer tissue. Positive WB detected in mouse brain tissue. Observed molecular weight by Western-blot: 60 kDa
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
KIAA0341 antibody; N4BP3 antibody; Nedd4 binding protein 3 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of N4BP3 recombinant protein (Accession Number: XM_047416997). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:113007(N4BP3 Antibody) at dilution of 1:600
Immunohistochemistry of paraffin-embedded human kidney tissue slide using Catalog No:113007(N4BP3 Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human kidney tissue slide using Catalog No:113007(N4BP3 Antibody) at dilution of 1:50 (under 40x lens)
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Background
N4BP3, also named as KIAA0341, a Fezzin family member, during nerve cell development. It is crucial for axonal and dendritic branching in developing neurons. It binds the protein NEDD4 and interacts with 14-3-3 proteins. N4BP3 is present in early neurites, growth cones, axons and dendrites of developing primary hippocampal neurons. This antibody detects 60 kDa N4BP3 protein and a weaker 40 kDa protein (unknown).
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