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Product Name
Myoglobin antibody
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Description
Myoglobin Mouse Monoclonal antibody. Positive IHC detected in human heart tissue. Positive WB detected in human heart tissue. Observed molecular weight by Western-blot: 17-18 kDa
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Tested applications
ELISA, IHC, WB
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Species reactivity
Human; other species not tested.
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Alternative names
MYO antibody; myoglobin antibody
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Isotype
Mouse IgG1
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Preparation
This antibody was obtained by immunization of Myoglobin recombinant protein (Accession Number: XM_005261605). Purification method: Protein G purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:5000-1:50000
IHC: 1:20-1:200
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Validations
human heart tissue were subjected to SDS PAGE followed by western blot with Catalog No:107317(MB Antibody) at dilution of 1:40000
Immunohistochemistry of paraffin-embedded human heart tissue slide using Catalog No:107317(MB Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human heart tissue slide using Catalog No:107317(MB Antibody) at dilution of 1:50 (under 40x lens)
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Background
Myoglobin is a cytoplasmic hemoprotein that is expressed primarily in cardiomyocytes and oxidative skeletal muscle fibers, functioning on facilitating oxygen transport and modulating nitric oxide homeostasis within cardiac and skeletal myocytes. Recent studies indicated that myoglobin was also expressed in non-muscle tissues. This antibody well recognized endogenous myoglobin in muscle lysates.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"