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Product Name
MYO1A antibody
- Documents
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Description
MYO1A Rabbit Polyclonal antibody. Positive IP detected in HeLa cells. Positive WB detected in mouse brain tissue, A431 cells, HeLa cells, HepG2 cells. Positive IHC detected in human small intestine tissue, human brain tissue, human kidney tissue, human lung tissue, human spleen tissue, human testis tissue. Positive IF detected in HepG2 cells, Hela cells. Observed molecular weight by Western-blot: 130 kDa
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Tested applications
ELISA, WB, IF, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
BBM I antibody; BBMI antibody; Brush border myosin I antibody; DFNA48 antibody; MIHC antibody; MYHL antibody; MYO1A antibody; Myosin I heavy chain antibody; myosin IA antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of MYO1A recombinant protein (Accession Number: NM_001256041). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:112982(MYO1A antibody) at dilution of 1:500
Immunohistochemistry of paraffin-embedded human small intestine tissue slide using Catalog No:112982(MYO1A Antibody) at dilution of 1:50 (under 40x lens)
Immunohistochemistry of paraffin-embedded human small intestine tissue slide using Catalog No:112982(MYO1A Antibody) at dilution of 1:50 (under 10x lens)
Immunofluorescent analysis of HepG2 cells, using MYO1A antibody Catalog No:112982 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-MYO1A (IP:Catalog No:112982, 4ug; Detection:Catalog No:112982 1:500) with HeLa cells lysate 3200ug.
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Background
MYO1A represents myosin IA
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