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Product Name
MYLK4 antibody
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Description
MYLK4 Rabbit Polyclonal antibody. Positive IHC detected in human tonsillitis tissue. Positive IP detected in HEK-293 cells. Positive WB detected in L02 cells, HEK-293 cells. Observed molecular weight by Western-blot: 45 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human; other species not tested.
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Alternative names
MYLK4 antibody; SgK085 antibody; Sugen kinase 85 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of MYLK4 recombinant protein (Accession Number: NM_001012418). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
L02 cells were subjected to SDS PAGE followed by western blot with Catalog No:112975(MYLK4 Antibody) at dilution of 1:1000
IP Result of anti-MYLK4 (IP:Catalog No:112975, 4ug; Detection:Catalog No:112975 1:500) with HEK-293 cells lysate 3600ug.
Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using Catalog No:112975(MYLK4 Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using Catalog No:112975(MYLK4 Antibody) at dilution of 1:50 (under 40x lens)
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Background
MYLK4(Myosin light chain kinase family member 4) is also named as SGK085 and belongs to the CAMK Ser/Thr protein kinase family. MLCKs are activated by Ca2+-calmodulin complexes and require Mg2+ or Ca2+ as a co-factor. It has 2 isoforms produced by alternative splicing.
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