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Product Name
MYH14 antibody
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Description
MYH14 Rabbit Polyclonal antibody. Positive WB detected in COLO 320 cells, human skeletal muscle tissue, mouse colon tissue, mouse kidney tissue. Positive IP detected in mouse kidney tissue. Positive IF detected in Hela cells. Observed molecular weight by Western-blot: 228 kDa
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Tested applications
ELISA, WB, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
MHC16 antibody; MYH14 antibody; myosin antibody; Myosin 14 antibody; Myosin heavy chain 14 antibody; myosin antibody; heavy chain 14 antibody; NMHC II C antibody; non-muscle II c antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_024729). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:1000-1:10000
IF: 1:10-1:100
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Validations
COLO 320 cells were subjected to SDS PAGE followed by western blot with Catalog No:112931(MYH14 antibody) at dilution of 1:800
Immunofluorescent analysis of Hela cells, using MYH14 antibody Catalog No:112931 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
IP Result of anti-MYH14 (IP:Catalog No:112931, 4ug; Detection:Catalog No:112931 1:2000) with mouse kidney tissue lysate 4800ug.
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Background
MYH14, also named as KIAA2034 and NMHC II-C, is cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. Defects in MYH14 are the cause of deafness autosomal dominant type 4 (DFNA4). The antibody is specific to MYH14.
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References
- Liu M, Yang J, Li M. Tanshinone IIA attenuates interleukin-17A-induced systemic sclerosis patient-derived dermal vascular smooth muscle cell activation via inhibition of the extracellular signal-regulated kinase signaling pathway. Clinics (São Paulo, Brazil). 70(4):250-6. 2015.
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