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MYCN antibody

Cat.#: 112918

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Product Information

  • Product Name
    MYCN antibody
  • Documents
  • Description
    MYCN Rabbit Polyclonal antibody. Positive IHC detected in human thyroid cancer tissue. Positive WB detected in SKOV-3 cells, C6 cells, mouse brain tissue, rat brain tissue, SH-SY5Y cells. Observed molecular weight by Western-blot: 67 kDa
  • Tested applications
    ELISA, WB, IHC
  • Species reactivity
    Human, Mouse, Rat; other species not tested.
  • Alternative names
    bHLHe37 antibody; MODED antibody; MYCN antibody; N myc antibody; N myc proto oncogene protein antibody; ODED antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of MYCN recombinant protein (Accession Number: XM_017004168). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IHC: 1:50-1:500

  • Validations

    SKOV-3 cells were subjected to SDS PAGE followed by western blot with Catalog No:112918(MYCN antibody) at dilution of 1:1000

    SKOV-3 cells were subjected to SDS PAGE followed by western blot with Catalog No:112918(MYCN antibody) at dilution of 1:1000

    Immunohistochemistry of paraffin-embedded human thyroid cancer tissue slide using Catalog No:112918(MYCN Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

    Immunohistochemistry of paraffin-embedded human thyroid cancer tissue slide using Catalog No:112918(MYCN Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

  • Background
    MYCN, also named as BHLHE37 and NMYC, is a transcription factor. It is primarily expressed in normal developing embryos and is thought to be critical in brain and other neural development. It is often amplified in human neuroblastomas. IR34A can suppress MYCN expression, it suggest MYCN has a role in cell growth. The expression of the MCYN is upregulated in a variety of human tumors, most frequently neuroblastoma, where the level of expression appears to increase as the tumor progresses.
  • References
    • Suganuma R, Wang LL, Sano H. Peripheral neuroblastic tumors with genotype-phenotype discordance: a report from the Children's Oncology Group and the International Neuroblastoma Pathology Committee. Pediatric blood & cancer. 60(3):363-70. 2013.
    • Wang LL, Suganuma R, Ikegaki N. Neuroblastoma of undifferentiated subtype, prognostic significance of prominent nucleolar formation, and MYC/MYCN protein expression: a report from the Children's Oncology Group. Cancer. 119(20):3718-26. 2013.
    • Cage TA, Chanthery Y, Chesler L. Downregulation of MYCN through PI3K Inhibition in Mouse Models of Pediatric Neural Cancer. Frontiers in oncology. 5:111. 2015.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"