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Product Name
MUC16,CA125 antibody
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Description
MUC16,CA125 Rabbit Polyclonal antibody. Positive IP detected in HeLa cells. Positive WB detected in HeLa cells. Positive IHC detected in human ovary tumor tissue. Observed molecular weight by Western-blot: 90-100 kDa
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Tested applications
ELISA, WB, IP, IHC
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Species reactivity
Human; other species not tested.
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Alternative names
CA 125 antibody; CA125 antibody; FLJ14303 antibody; MUC 16 antibody; MUC16 antibody; Mucin 16 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_024690). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:50-1:500
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:112889(MUC16,CA125 antibody) at dilution of 1:300
IP Result of anti-MUC16,CA125 (IP:Catalog No:112889, 4ug; Detection:Catalog No:112889 1:600) with HeLa cells lysate 1200ug.
Immunohistochemistry of paraffin-embedded human ovary tumor tissue slide using Catalog No:112889(MUC16,CA125 Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
Immunohistochemistry of paraffin-embedded human ovary tumor tissue slide using Catalog No:112889(MUC16,CA125 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
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Background
MUC16, also named as CA125, is thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. It is a serum marker that is used routinely in gynecologic practice to monitor patients with ovarian cancer. Most biochemical studies have concluded that MUC16 is a high molecular mass glycoprotein, although estimates of its size range from 200 to 2000 kDa with smaller “subunits” being described by some investigators. The antibody recognizes the C-term of MUC16.
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